I think I figured out how you lose perma-Jugg.

[jameslieb1]

You lose it by getting down twice in one round.

[itscanny]

The only way I know is by getting either to round 15 or past it, can't quite remember.

[koolkidfica]

no NGTzombies have gotton past round 15 and spiderbite still has it

[Tasty Applesauce]

I went up to 22 earlier and still had it so there's that.

[Xieon]

The OP says you get it by going down twice in one round. No one tested that?

I'll try it in a second, I've lost it so gotta get it back, and try.

[way2g00d]

To Op: Instead of just stating what you think, please present viable proof in future. Thanks for your suggestions. :)

[AntJac]

Just confirmed round 15 with no downs and I lost it.

[Chopper]

I know that it can be lost on round 15, as it happened to me in a coop game today, and the other people in our party said the same thing for there game. Can post a video if necessary.

[Megaton A]

I have done some testing on this, and I have gained and lost it multiple times. Every single time I lost it was at the beginning of round 15, and every single time I had it at round 15, I lost it.

However, I have had occasions where I'm sure I did NOT have it, but still only got redscreen on my third hit instead of going down on the second. I have also gone down by getting hit twice without it on Die Rise. I think this is where some of the confusion comes from. I'm guessing it occasionally just glitches for whatever reason and gives you the effect of Persistajugg even when you don't have it. It's either that, or they changed how the damage is calculated and you take less damage if the zombie is just at arm's reach (as if they're barely scratching you) but I highly doubt that it the case.

What I can say for sure is, in my experience, 100% of the time that I have lost it was just as round 15 started, and 100% of the time I started round 15 with it, I lost it. I have also gone down multiple times in a game and even a round without losing it.

Edit; I've heard the reason is that the body armor zombies do slightly less damage. I can't confirm this, but it makes sense.

[83457]

Iirc, you lose it on 15, but you still have it until the next game starts, no?

So if you went down on 20, you would still take 4 hits, but next game, it is gone.

I also need to check this, but I am at work...

[Chopper]

I'm adamant that regardless of what sound you get it, you don't lose it until you go down.

I've played so much Die Rise now that I realise there is a distinct difference between kiting before the first down, and kiting after.

Before the first down, I can stay a fair bit closer to the pack. 2 quick hits can be ignored and you continue your path. Sometimes you get a rd and 4th, sometimes not. The key is that with perma jugg you can take that 3rd and 4th hit, and then know to move away from the pack.

Without perma jugg I always will move away from the pack after 2 hits, as those 2nd 2 can come so quickly and then you are on a red screen.

[Matuzz]

I'm pretty sure you will lose it when you go down after round 14.

[83457]

I can confirm that I got the sound at 15, but in the 20's, i easily took 4 hits at once and was fine.

Also in who's who mode, you don't have it.

[Chopper]

Ok 100% confirmed that you keep it past 15 until you go down.

[perfectlemonade]

Ok 100% confirmed that you keep it past 15 until you go down.

I recall you yourself confirming this with that "One Chance in China Room" challenge you did a while back. ;)

[Chopper]

I'd forgotten about that mate! Well, double and triple confirmed then from gaming tonight :)